{"product_id":"transcription-competent-bundibugyo-ebolavirus-vlps-encoding-nluc-minigenome","title":"Transcription Competent Bundibugyo Ebolavirus VLPs Encoding nLuc Minigenome","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAvailability\u003c\/span\u003e: \u003c\/strong\u003eExpected to ship in September 2026.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eDescription:\u003c\/b\u003e This transcription-competent virus-like particle is comprised of the Bundibugyo ebolavirus Matrix protein VP40, the secondary Matrix protein VP24, the ebolavirus Glycoprotein GP, and the ebolavirus Nucleoprotein NP. The VLP encapsidates an ebolavirus minigenome expressing the reporter protein nano Luciferase (nLuc), as well as the other proteins of the Ribonucleprotein complex (Polymerase protein L, Transcription Activator protein VP30, and Polymerase cofactor protein VP35). \u003cbr\u003e\u003cbr\u003e\u003cb\u003eStrain History:\u003c\/b\u003e Bundibugyo ebolavirus emerged as a distinct species of ebolavirus during a 2007 outbreak in western Uganda. The genomic sequence in \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/NC_014373.1\"\u003eavailable in NCBI\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003ePassage History:\u003c\/b\u003e Vero E6 (2). References (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC4189044\/\"\u003e1\u003c\/a\u003e\u003ca href=\"https:\/\/www.cabidigitallibrary.org\/doi\/full\/10.5555\/19612202523\"\u003e\u003c\/a\u003e) and (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC2581435\/\"\u003e2\u003c\/a\u003e).\u003cbr\u003e\u003cbr\u003e\u003cb\u003eProduction:\u003c\/b\u003e Co-transfection of plasmids expressing the nLuc minigenome, structural proteins (VP40, GP, NP, VP24) and ribonucleoprotein complex (L, VP30, VP35) into human embryonic kidney 293T (HEK 293T) cells. Collected supernatant purified and concentrated by sucrose gradient centrifugation.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eBiosafety Level:\u003c\/b\u003e 2\u003cbr\u003e\u003cbr\u003e\u003cb\u003eFormulation:\u003c\/b\u003e 1.0 mg of protein suspended in PBS.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e 1.0 mL\/vial. Purified and concentrated cell culture supernatant frozen at -80°C. Delivered on dry ice. Additional kit components include L, VP30, VP35 and NP protein expressing plasmids.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eInstructions For Use:\u003c\/b\u003e Prior to infection of cells with VLPs, transfect cells for 24-48 hours with the L, VP30, VP35 and NP protein expressing plasmids provided in the kit using a suitable transfection reagent.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eQA\/QC:\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eProtein concentration\u003c\/li\u003e\n\u003cli\u003eTitration by nLuc signal quantification following VLP infection.\u003c\/li\u003e\n\u003cli\u003eMycoplasma test by PCR\u003c\/li\u003e\n\u003cli\u003eSterility test for bacterial and fungal contamination\u003c\/li\u003e\n\u003cli\u003eEndotoxin test \u0026lt;0.25 EU\/mL\u003c\/li\u003e\n\u003cli\u003eElectron microscopy imaging to confirm VLP structure\u003c\/li\u003e\n\u003cli\u003eDocumentation: Experimental conditions for QC tests\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003e\u003cu\u003eProduction Batch Information links (downloadable):\u003c\/u\u003e\u003c\/b\u003e\u003cbr\u003e\u003cbr\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/Placeholder_PDF_for_product_pages_1.pdf?v=1779819181\" target=\"_blank\"\u003eCertificate of Analysis\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/Placeholder_PDF_for_product_pages_1.pdf?v=1779819181\" target=\"_blank\"\u003eBatch Record: Detailed Production and QC Data\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV_Product_Use_Policy_04.10.2025.pdf.pdf?v=1746046086\" target=\"_blank\"\u003eProduct Use Policy and Material Transfer Agreement\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eEbolavirus Minigenome Construction:\u003c\/b\u003e The\u003cspan class=\"citation-23 citation-end-23\"\u003e negative-sense ebolavirus minigenome encoding nLuc \u003c\/span\u003e\u003cspan class=\"citation-22\"\u003econsists of the authentic viral \u003c\/span\u003e\u003cspan class=\"citation-22\"\u003eleader\u003c\/span\u003e\u003cspan class=\"citation-22\"\u003e and \u003c\/span\u003e\u003cspan class=\"citation-22\"\u003etrailer UTR sequences\u003c\/span\u003e\u003cspan class=\"citation-22 citation-end-22\"\u003e at the 3' and 5' ends, which retain the essential cis-acting promoter signals required for genome transcription.\u003csup class=\"superscript\"\u003e\u003c!----\u003e\u003c\/sup\u003e\u003c\/span\u003e \u003cspan class=\"citation-21\"\u003eSandwiched between these terminal UTRs is a negative-sense nLuc open reading frame \u003c\/span\u003e\u003cspan class=\"citation-21 citation-end-21\"\u003eflanked by NP gene 5' UTR start and L gene 3' UTR end sequences\u003c\/span\u003e\u003cspan class=\"citation-20 citation-end-20\"\u003e.\u003c\/span\u003e For additional information, see references (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC5654650\/\"\u003e1\u003c\/a\u003e) and (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC5660472\/\"\u003e2\u003c\/a\u003e).\u003cbr\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eAdditional Strains:\u003c\/b\u003e If you require a VLP for a different ebolavirus strain, please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e. We can produce VLPs from multiple ebolavirus strains to meet your needs.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAdditional Viruses Available:\u003c\/b\u003e If you are looking for a virus species or strain not listed on our website, please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e with your request. Our team of experts will work with you to create the product that meets your research needs and budget.\u003c\/p\u003e","brand":"Advanced Virology Inc.","offers":[{"title":"Default Title","offer_id":45290469458035,"sku":null,"price":1500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/VLP---EGFP_nanoLuc.png?v=1783628897","url":"https:\/\/advancedvirology.com\/products\/transcription-competent-bundibugyo-ebolavirus-vlps-encoding-nluc-minigenome","provider":"Advanced Virology Inc.","version":"1.0","type":"link"}