{"product_id":"venezuelan-equine-encephalitis-virus-vaccine-strain-tc-83-copy","title":"Venezuelan Equine Encephalitis Virus Vaccine Strain TC-83 eGFP Reporter","description":"\u003cp\u003e\u003cb\u003eDescription:\u003c\/b\u003e This alphavirus expresses the reporter protein eGFP at very high levels with minimal effect on viral replication or virulence.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eStrain History:\u003c\/b\u003e TC-83 is a live-attenuated vaccine strain of Venezuelan equine encephalitis virus (VEEV) derived from the virulent clade IA\/B epizootic strain Trinidad donkey (donkey, Trinidad, 1983). The genomic sequence in \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/L01443.1\"\u003eavailable in NCBI\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003ePassage History:\u003c\/b\u003e Guinea pig heart cells (83). Reference (\u003ca href=\"https:\/\/www.cabidigitallibrary.org\/doi\/full\/10.5555\/19612202523\"\u003e1\u003c\/a\u003e).\u003cbr\u003e\u003cbr\u003e\u003cb\u003eProduction:\u003c\/b\u003e Transfection of \u003ci\u003ein vitro\u003c\/i\u003e transcribed viral genomic RNA into baby hamster kidney (BHK) cells. No serial passages performed.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eBiosafety Level:\u003c\/b\u003e 2\u003cbr\u003e\u003cbr\u003e\u003cb\u003eTiter:\u003c\/b\u003e 1.00 x 10\u003csup\u003e9\u003c\/sup\u003e TCID50\/mL on BHK cells.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e 1.0 mL\/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eQA\/QC:\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInfectious titer in TCID50\/mL \u003c\/li\u003e\n\u003cli\u003eWhole-genome sequencing by NGS\u003c\/li\u003e\n\u003cli\u003eMycoplasma test by PCR\u003c\/li\u003e\n\u003cli\u003eSterility test for bacterial and fungal contamination\u003c\/li\u003e\n\u003cli\u003eEndotoxin test \u0026lt;0.25 EU\/mL\u003c\/li\u003e\n\u003cli\u003eDocumentation: Experimental conditions for QC tests\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003eReporter Virus Construction:\u003c\/b\u003e Reporter protein coding sequences were inserted between the capsid and PE2 loci and contain the 2A-like protease sequence from the Thosea asigna virus (TaV site) at the 3’ end. Upon polyprotein translation in infected cells, the capsid autoprotease domain and the TaV protease cleave the reporter protein from the capsid, resulting in reporter expression independent of incorporation into the virus particle. For additional information, see \u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3911548\/\"\u003ereference\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cu\u003eProduction Batch Information links (downloadable):\u003c\/u\u003e\u003c\/b\u003e\u003cbr\u003e\u003cbr\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV-VR-007_-_Certificate_of_Analysis_-_Signed.pdf?v=1781126077\" target=\"_blank\"\u003eCertificate of Analysis\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV-VR-007_-_Sequencing_Results.pdf?v=1781126077\" rel=\"noopener\" target=\"_blank\"\u003eNGS Results and Complete Genome Sequence\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV-VR-007_-_Batch_Record.pdf?v=1781126078\" target=\"_blank\"\u003eBatch Record: Detailed Production and QC Data\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV_Product_Use_Policy_04.10.2025.pdf.pdf?v=1746046086\" target=\"_blank\"\u003eProduct Use Policy and Material Transfer Agreement\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAdditional Formats:\u003c\/b\u003e This product can be made as a purified or inactivated virus stock, or as purified viral genomic material. Many different reporter proteins and molecular tags can be incorporated. \u003ca href=\"https:\/\/advancedvirology.com\/pages\/made-to-order-viruses\"\u003eCustom variant design and production services\u003c\/a\u003e are also available. Please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e for additional information.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAdditional Strains:\u003c\/b\u003e If you require a strain of this virus not listed on our website, please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e. We can produce a wide range of common strains to meet your needs.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAdditional Viruses Available:\u003c\/b\u003e If you are looking for a virus species or strain not listed on our website, please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e with your request. Our team of experts will work with you to create the product that meets your research needs and budget.\u003c\/p\u003e","brand":"Advanced Virology Inc.","offers":[{"title":"Default Title","offer_id":45089825194099,"sku":"AV-VR-007","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/Alphavirus_-_eGFP_Reporter.png?v=1764690198","url":"https:\/\/advancedvirology.com\/products\/venezuelan-equine-encephalitis-virus-vaccine-strain-tc-83-copy","provider":"Advanced Virology Inc.","version":"1.0","type":"link"}