{"product_id":"yellow-fever-virus-vaccine-strain-17d-egfp-reporter-copy","title":"Yellow Fever Virus Vaccine Strain 17D mCherry Reporter","description":"\u003cp\u003e\u003cb\u003eDescription:\u003c\/b\u003e This flavivirus expresses the reporter protein mCherry.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eStrain History:\u003c\/b\u003e 17D-204 is the live-attenuated vaccine strain of yellow fever virus (YFV) derived from the virulent human clinical isolate Asibi strain (Ghana, 1927). The genomic sequence is \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/nucleotide\/NC_002031.1\"\u003eavailable in NCBI\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003ePassage History:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eParental Asibi strain: Rhesus macaque monkeys (53), intermittent passages in \u003cem\u003eAedes aegypti\u003c\/em\u003e mosquitoes (Unknown). References (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC304575\/\"\u003e1\u003c\/a\u003e) and (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8310116\/\"\u003e2\u003c\/a\u003e).\u003c\/li\u003e\n\u003cli\u003e17D-204: Mouse embryo tissue (18), minced whole chick embryo (58), minced whole chick embryo without brain and spinal cord (128). References (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3883177\/\"\u003e3\u003c\/a\u003e) and (\u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC5563254\/\"\u003e4\u003c\/a\u003e).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003eProduction: \u003c\/b\u003eTransfection of \u003ci\u003ein vitro\u003c\/i\u003e transcribed viral genomic RNA into Vero cells. No serial passages performed.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eBiosafety Level:\u003c\/b\u003e 2\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTiter:\u003c\/strong\u003e 3.16 x 10\u003csup\u003e6\u003c\/sup\u003e TCID50\/mL on Vero cells.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eFormat:\u003c\/b\u003e 1.0 mL\/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eQA\/QC:\u003c\/b\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInfectious titer in TCID50\/mL\u003c\/li\u003e\n\u003cli\u003eWhole-genome sequencing by NGS\u003c\/li\u003e\n\u003cli\u003eMycoplasma test by PCR\u003c\/li\u003e\n\u003cli\u003eSterility test for bacterial and fungal contamination\u003c\/li\u003e\n\u003cli\u003eEndotoxin test \u0026lt;0.25 EU\/mL\u003c\/li\u003e\n\u003cli\u003eDocumentation: Experimental conditions for QC tests.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cb\u003eReporter Virus Construction:\u003c\/b\u003e Reporter protein coding sequences were inserted between the 5' UTR and the capsid, and contain the first 75 nucleotides of the capsid protein at the 5' end (modified with silent mutations to prevent homologous recombination with the capsid gene) and the 2A-like protease sequence from the Thosea asigna virus (TaV site) at the 3’ end. Upon polyprotein translation in infected cells, the TaV protease cleaves the reporter protein from the capsid, resulting in reporter expression independent of incorporation into the virus particle. For additional information, see \u003ca href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC7599567\/\"\u003ereference\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cu\u003eProduction Batch Information links (downloadable):\u003c\/u\u003e\u003c\/b\u003e\u003cbr\u003e\u003cbr\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV-VR-006_-_Certificate_of_Analysis_-_Signed.pdf?v=1781040150\" target=\"_blank\"\u003eCertificate of Analysis\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV-VR-006_-_Sequencing_Results.pdf?v=1781040150\" rel=\"noopener\" target=\"_blank\"\u003eNGS Results and Complete Genome Sequence\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV-VR-006_-_Batch_Record.pdf?v=1781040150\" target=\"_blank\"\u003eBatch Record: Detailed Production and QC Data\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/AV_Product_Use_Policy_04.10.2025.pdf.pdf?v=1746046086\" target=\"_blank\"\u003eProduct Use Policy and Material Transfer Agreement\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAdditional Formats:\u003c\/b\u003e This product can be made as a purified or inactivated virus stock, or as purified viral genomic material. Many different reporter proteins and molecular tags can be incorporated. \u003ca href=\"https:\/\/advancedvirology.com\/pages\/made-to-order-viruses\"\u003eCustom variant design and production services\u003c\/a\u003e are also available. Please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e for additional information.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAdditional Strains:\u003c\/b\u003e If you require a strain of this virus not listed on our website, please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e. We can produce a wide range of common strains to meet your needs.\u003cbr\u003e\u003cbr\u003e\u003cb\u003eAdditional Viruses Available:\u003c\/b\u003e If you are looking for a virus species or strain not listed on our website, please \u003ca href=\"https:\/\/advancedvirology.com\/pages\/contact-us\"\u003econtact us\u003c\/a\u003e with your request. Our team will work with you to create the product that meets your research needs and budget.\u003c\/p\u003e","brand":"Advanced Virology Inc.","offers":[{"title":"Default Title","offer_id":45087935037555,"sku":"AV-VR-006","price":550.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0615\/7018\/7379\/files\/Flavivirus_-_Chimeric_and_Repoter_eGFP.png?v=1764689824","url":"https:\/\/advancedvirology.com\/products\/yellow-fever-virus-vaccine-strain-17d-egfp-reporter-copy","provider":"Advanced Virology Inc.","version":"1.0","type":"link"}