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Sindbis Virus Strain TR339 eGFP Reporter
$500.00
Unit price
/
Unavailable
Sindbis Virus Strain TR339 eGFP Reporter is backordered and will ship as soon as it is back in stock.
To support our goal of supplying experiment-ready volumes of virus to our customers, we offer volume-based pricing. Adjusted pricing is applied during checkout.
- Purchase of one vial - list price
- Purchase of two vials - 10% off order
- Purchase of three vials - 20% off order
- Purchase of four or more vials - 30% off order
- For large orders, contact us for a custom quote.
- Academic customers - 60% off order.
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Description: Wild-type alphavirus expressing the reporter protein eGFP at very high levels with minimal effect on viral replication or virulence.
Strain History: TR339 is the consensus wild-type strain of Sindbis virus (SINV) derived from the prototype AR339 isolate (Culex spp. mosquitoes, Egypt, 1952). The genomic sequence is available in NCBI.
Passage History: Suckling mouse brain (8). References (1) and (2)
Production: Transfection of in vitro transcribed viral genomic RNA into baby hamster kidney (BHK) cells. No serial passages performed.
Biosafety Level: 2
Titer: At least 1 x 107 PFU/mL on BHK cells.
Format: 1.0 mL/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.
QA/QC:
Reporter Virus Construction: Reporter protein coding sequences were inserted between the capsid and PE2 loci and contain the 2A-like protease sequence from the Thosea asigna virus (TaV site) at the 3’ end. Upon polyprotein translation in infected cells, the capsid autoprotease domain and the TaV protease cleave the reporter protein from the capsid, resulting in reporter expression independent of incorporation into the virus particle. For additional information, see reference.
Shipping: As this product is a Sindbis virus strain, customers must submit a USDA APHIS permit VS 16-3 to Advanced Virology prior to order shipment. The permit and additional information are available at USDA APHIS Permits.
Production Batch Information links (downloadable):
Certificate of Analysis
NGS Results and Complete Genome Sequence
Batch Record: Detailed Production and QC Data
Product Use Policy and Material Transfer Agreement
Additional Formats: This product can be made as a purified or inactivated virus stock, or as purified viral genomic material. Many different reporter proteins and molecular tags can be incorporated. Custom variant design and production services are also available. Please contact us for additional information.
Additional Strains: If you require a strain of this virus not listed on our website, please contact us. We can produce a wide range of common strains to meet your needs.
Additional Viruses Available: If you are looking for a virus species or strain not listed on our website, please contact us with your request. Our team of experts will work with you to create the product that meets your research needs and budget.
Strain History: TR339 is the consensus wild-type strain of Sindbis virus (SINV) derived from the prototype AR339 isolate (Culex spp. mosquitoes, Egypt, 1952). The genomic sequence is available in NCBI.
Passage History: Suckling mouse brain (8). References (1) and (2)
Production: Transfection of in vitro transcribed viral genomic RNA into baby hamster kidney (BHK) cells. No serial passages performed.
Biosafety Level: 2
Titer: At least 1 x 107 PFU/mL on BHK cells.
Format: 1.0 mL/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.
QA/QC:
- Titer in PFU/mL by plaque assay
- Genome equivalents/mL by qPCR
- Whole-genome sequencing by NGS
- Mycoplasma test by PCR
- Sterility test for bacterial and fungal contamination
- Endotoxin test <10 EU/mL
- Documented expression of eGFP reporter protein
- Documentation: Experimental conditions for QC tests, documentation of all reagents used.
Reporter Virus Construction: Reporter protein coding sequences were inserted between the capsid and PE2 loci and contain the 2A-like protease sequence from the Thosea asigna virus (TaV site) at the 3’ end. Upon polyprotein translation in infected cells, the capsid autoprotease domain and the TaV protease cleave the reporter protein from the capsid, resulting in reporter expression independent of incorporation into the virus particle. For additional information, see reference.
Shipping: As this product is a Sindbis virus strain, customers must submit a USDA APHIS permit VS 16-3 to Advanced Virology prior to order shipment. The permit and additional information are available at USDA APHIS Permits.
Production Batch Information links (downloadable):
Certificate of Analysis
NGS Results and Complete Genome Sequence
Batch Record: Detailed Production and QC Data
Product Use Policy and Material Transfer Agreement
Additional Formats: This product can be made as a purified or inactivated virus stock, or as purified viral genomic material. Many different reporter proteins and molecular tags can be incorporated. Custom variant design and production services are also available. Please contact us for additional information.
Additional Strains: If you require a strain of this virus not listed on our website, please contact us. We can produce a wide range of common strains to meet your needs.
Additional Viruses Available: If you are looking for a virus species or strain not listed on our website, please contact us with your request. Our team of experts will work with you to create the product that meets your research needs and budget.