SINV TR339/EEEV FL93-939 Chimeric NanoLuciferase Reporter Virus
To support our goal of supplying experiment-ready volumes of virus to our customers, we offer volume-based pricing. Adjusted pricing is applied during checkout.
- Purchase of one vial - list price
- Purchase of two vials - 10% off order
- Purchase of three vials - 20% off order
- Purchase of four or more vials - 30% off order
- For large orders, contact us for a custom quote.
- Academic customers - 60% off order.
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Description: This virus is a chimeric alphavirus containing the non-structural proteins of Sindbis virus (SINV) strain TR339 and the structural proteins from Eastern equine encephalitis virus (EEEV) strain FL93-939. Additionally, this virus expresses the reporter protein NanoLuciferase (nLuc) at very high levels with a minimal effect on viral replication or virulence. This product allows the study of EEEV FL93-939 in a non Select Agent BSL-2 laboratory setting.
Strain History: TR339 is the consensus wild-type strain of SINV derived from the prototype AR339 isolate (Culex spp. mosquitoes, Egypt, 1952). The genomic sequence is available in NCBI. EEEV strain FL93-939 was isolated from an infected mosquito pool (Culiseta melanura) in Florida in 1993. The genomic sequence is available in NCBI.
Passage History:
- SINV TR339: Mosquito and suckling mice (unknown), CEF (unknown), BHK (unknown). References (1), (2) and (3).
- EEEV FL93-939: Vero (Unknown), newborn mouse brain (1). References (1) and (2).
Production: Transfection of In-Vitro Transcribed viral genomic RNA into Baby Hamster Kidney (BHK) cells. No serial passages performed.
Biosafety Level: 2
Titer: At least 1x10^7 PFU/mL on BHK cells. For lot titer, see the Certificate of Analysis below.
Format: Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.
QA/QC:
- Titer in PFU/mL by plaque assay
- Genome copy number/mL by qPCR
- Whole-genome sequencing by NGS
- Mycoplasma test by PCR
- Sterility test for bacterial contamination
- Endotoxin test <10 EU/mL
- Documented expression of nLuc reporter protein
- Documentation: Cell culture growth and passage, experimental conditions for QC tests, documentation of all reagents used.
Reporter virus construction: Reporter protein coding sequences were inserted between the capsid and PE2 loci and contain the 2A-like protease sequence from the Thosea asigna virus (TaV site) at the 3’ end. Upon polyprotein translation in infected cells, the capsid autoprotease domain and the TaV protease cleave the reporter protein from the capsid, resulting in reporter expression independent of incorporation into the virus particle. For additional information, see Reference.
Production Batch Information links (downloadable):
