SINV TR339/EEEV FL93-939 Chimeric Virus

Description: This virus is a chimeric alphavirus containing the non-structural proteins of Sindbis virus (SINV) strain TR339 and the structural proteins from Eastern equine encephalitis virus (EEEV) strain FL93-939. This product allows the study of EEEV FL93-939 in a non Select Agent BSL-2 laboratory setting.

Strain History: TR339 is the consensus wild-type strain of SINV derived from the prototype AR339 isolate (Culex spp. mosquitoes, Egypt, 1952). The genomic sequence is available in NCBI. EEEV strain FL93-939 was isolated from an infected mosquito pool (Culiseta melanura) in Florida in 1993. The genomic sequence is available in NCBI.

Passage History:

• SINV TR339: Mosquito and suckling mice (unknown), CEF (unknown), BHK (unknown). References (1), (2) and (3).
• EEEV FL93-939: Vero (Unknown), newborn mouse brain (1). References (1) and (2).

Production: Transfection of In-Vitro Transcribed viral genomic RNA into Baby Hamster Kidney (BHK) cells. No serial passages performed.

Biosafety Level: 2

Titer: 1.78 x 10^9 PFU/mL on BHK cells.

Format: 1.0 mL/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice. 

QA/QC:

• Titer in PFU/mL by plaque assay
• Genome equivalents/mL by qPCR
• Whole-genome sequencing by NGS
• Mycoplasma test by PCR
• Sterility test for bacterial and fungal contamination
• Endotoxin test <10 EU/mL
• Documentation: Experimental conditions for QC tests, documentation of all reagents used.

Shipping: As this product is a Sindbis virus derivative, customers must submit a USDA APHIS permit VS 16-3 to Advanced Virology prior to order shipment. The permit and additional information is available at USDA APHIS Permits.

Production Batch Information links (downloadable):

Certificate of Analysis

NGS Results and Complete Genome Sequence

Batch Record: Detailed Production and QC Data

Product Use Policy and Material Transfer Agreement