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SINV TR339/EEEV FL93-939 Chimeric Virus

$450.00

To support our goal of supplying experiment-ready volumes of virus to our customers, we offer volume-based pricing. Adjusted pricing is applied during checkout.

  • Purchase of one vial - list price
  • Purchase of two vials - 10% off order
  • Purchase of three vials - 20% off order
  • Purchase of four or more vials - 30% off order
  • For large orders, contact us for a custom quote.
  • Academic customers - 60% off order.

Description: This virus is a chimeric alphavirus containing the non-structural proteins of Sindbis virus (SINV) strain TR339 and the structural proteins from Eastern equine encephalitis virus (EEEV) strain FL93-939. This product allows the study of EEEV FL93-939 in a non Select Agent BSL-2 laboratory setting.

Strain History: TR339 is the consensus wild-type strain of SINV derived from the prototype AR339 isolate (Culex spp. mosquitoes, Egypt, 1952). The genomic sequence is available in NCBI. EEEV strain FL93-939 was isolated from an infected mosquito pool (Culiseta melanura) in Florida in 1993. The genomic sequence is available in NCBI.

Passage History:

  • SINV TR339: Mosquito and suckling mice (unknown), CEF (unknown), BHK (unknown). References (1), (2) and (3).
  • EEEV FL93-939: Vero (Unknown), newborn mouse brain (1). References (1) and (2).

Production: Transfection of In-Vitro Transcribed viral genomic RNA into Baby Hamster Kidney (BHK) cells. No serial passages performed.

Biosafety Level: 2

Titer: At least 1x10^7 PFU/mL on BHK cells. For lot titer, see the Certificate of Analysis below.

Format: Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice. 

QA/QC:

  • Titer in PFU/mL by plaque assay
  • Genome copy number/mL by qPCR
  • Whole-genome sequencing by NGS
  • Mycoplasma test by PCR
  • Sterility test for bacterial contamination
  • Endotoxin test <10 EU/mL
  • Documentation: Cell culture growth and passage, experimental conditions for QC tests, documentation of all reagents used.

Production Batch Information links (downloadable):

Complete Genome Sequence

Certificate of Analysis

Detailed Production and QC Data

Product Use Policy and Material Transfer Agreement