SINV TR339/VEEV INH-9813 Chimeric Virus

$450.00

To support our goal of supplying experiment-ready volumes of virus to our customers, we offer volume-based pricing. Adjusted pricing is applied during checkout.

Purchase of one vial - list price
Purchase of two vials - 10% off order
Purchase of three vials - 20% off order
Purchase of four or more vials - 30% off order
For large orders, contact us for a custom quote.
Academic customers - 60% off order.

Description: This virus is a chimeric alphavirus containing the non-structural proteins of Sindbis virus (SINV) strain TR339 and the structural proteins from Venezuelan equine encephalitis virus (VEEV) strain INH-9813. This product allows the study of VEEV INH-9813 in a non Select Agent BSL-2 laboratory setting.

Strain History: TR339 is the consensus wild-type strain of SINV derived from the prototype AR339 isolate (Culex spp. mosquitoes, Egypt, 1952). The genomic sequence is available in NCBI. VEEV Type IC strain INH-9813 was isolated from human serum collected during the 1995 VEEV epidemic. The genomic sequence is available in NCBI.

Note: VEEV strain INH-9813 was found to contain a cell culture-adaptive mutation from glutamic acid to lysine at position 3 of the E2 protein (E3K) that likely confers a heparan sulfate binding phenotype (PubMed Reference).

Passage History:

SINV TR339: Mosquito and suckling mice (unknown), CEF (unknown), BHK (unknown). References (1), (2) and (3).
VEEV INH-9813: Vero (1). Reference (1).

Production: Transfection of In-Vitro Transcribed viral genomic RNA into Baby Hamster Kidney (BHK) cells. No serial passages performed.

Biosafety Level: 2

Titer: 3.00 x 10^7 PFU/mL on BHK cells.

Format: 1.0 mL/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.

QA/QC:

Titer in PFU/mL by plaque assay
Genome equivalents/mL by qPCR
Whole-genome sequencing by NGS
Mycoplasma test by PCR
Sterility test for bacterial and fungal contamination
Endotoxin test <10 EU/mL
Documentation: Experimental conditions for QC tests, documentation of all reagents used.

Shipping: As this product is a Sindbis virus derivative, customers must submit a USDA APHIS permit VS 16-3 to Advanced Virology prior to order shipment. The permit and additional information is available at USDA APHIS Permits.

Production Batch Information links (downloadable):

Batch Record: Detailed Production and QC Data

Product Use Policy and Material Transfer Agreement

SINV TR339/VEEV INH-9813 Chimeric Virus

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