Transcription Competent Bundibugyo Ebolavirus VLPs Encoding nLuc Minigenome

Availability: Expected to ship in September 2026.

Description: This transcription-competent virus-like particle is comprised of the Bundibugyo ebolavirus Matrix protein VP40, the secondary Matrix protein VP24, the ebolavirus Glycoprotein GP, and the ebolavirus Nucleoprotein NP. The VLP encapsidates an ebolavirus minigenome expressing the reporter protein nano Luciferase (nLuc), as well as the other proteins of the Ribonucleprotein complex (Polymerase protein L, Transcription Activator protein VP30, and Polymerase cofactor protein VP35). 

Strain History: Bundibugyo ebolavirus emerged as a distinct species of ebolavirus during a 2007 outbreak in western Uganda. The genomic sequence in available in NCBI.

Passage History: Vero E6 (2). References (1) and (2).

Production: Co-transfection of plasmids expressing the nLuc minigenome, structural proteins (VP40, GP, NP, VP24) and ribonucleoprotein complex (L, VP30, VP35) into human embryonic kidney 293T (HEK 293T) cells. Collected supernatant purified and concentrated by sucrose gradient centrifugation.

Biosafety Level: 2

Formulation: 1.0 mg of protein suspended in PBS.

Format: 1.0 mL/vial. Purified and concentrated cell culture supernatant frozen at -80°C. Delivered on dry ice. Additional kit components include L, VP30, VP35 and NP protein expressing plasmids.

Instructions For Use: Prior to infection of cells with VLPs, transfect cells for 24-48 hours with the L, VP30, VP35 and NP protein expressing plasmids provided in the kit using a suitable transfection reagent.

QA/QC:

• Protein concentration
• Titration by nLuc signal quantification following VLP infection.
• Mycoplasma test by PCR
• Sterility test for bacterial and fungal contamination
• Endotoxin test <0.25 EU/mL
• Electron microscopy imaging to confirm VLP structure
• Documentation: Experimental conditions for QC tests

Production Batch Information links (downloadable):

Certificate of Analysis

Batch Record: Detailed Production and QC Data

Product Use Policy and Material Transfer Agreement

Ebolavirus Minigenome Construction: The negative-sense ebolavirus minigenome encoding nLuc consists of the authentic viral leader and trailer UTR sequences at the 3' and 5' ends, which retain the essential cis-acting promoter signals required for genome transcription. Sandwiched between these terminal UTRs is a negative-sense nLuc open reading frame flanked by NP gene 5' UTR start and L gene 3' UTR end sequences. For additional information, see references (1) and (2).

Additional Strains: If you require a VLP for a different ebolavirus strain, please contact us. We can produce VLPs from multiple ebolavirus strains to meet your needs.

Additional Viruses Available: If you are looking for a virus species or strain not listed on our website, please contact us with your request. Our team of experts will work with you to create the product that meets your research needs and budget.