Venezuelan Equine Encephalitis Virus Vaccine Strain TC-83 mCherry Reporter

Description: This alphavirus expresses the reporter protein mCherry at very high levels with minimal effect on viral replication or virulence.

Strain History: TC-83 is a live-attenuated vaccine strain of Venezuelan equine encephalitis virus (VEEV) derived from the virulent clade IA/B epizootic strain Trinidad donkey (donkey, Trinidad, 1983). The genomic sequence in available in NCBI.

Passage History: Guinea pig heart cells (83). Reference (1).

Production: Transfection of in vitro transcribed viral genomic RNA into baby hamster kidney (BHK) cells. No serial passages performed.

Biosafety Level: 2

Titer: 1.00 x 10⁹ TCID50/mL on BHK cells.

Format: 1.0 mL/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.

QA/QC:

• Infectious titer in TCID50/mL 
• Whole-genome sequencing by NGS
• Mycoplasma test by PCR
• Sterility test for bacterial and fungal contamination
• Endotoxin test <0.25 EU/mL
• Documentation: Experimental conditions for QC tests

Reporter Virus Construction: Reporter protein coding sequences were inserted between the capsid and PE2 loci and contain the 2A-like protease sequence from the Thosea asigna virus (TaV site) at the 3’ end. Upon polyprotein translation in infected cells, the capsid autoprotease domain and the TaV protease cleave the reporter protein from the capsid, resulting in reporter expression independent of incorporation into the virus particle. For additional information, see reference.

Production Batch Information links (downloadable):

Certificate of Analysis

NGS Results and Complete Genome Sequence

Batch Record: Detailed Production and QC Data

Product Use Policy and Material Transfer Agreement

Additional Formats: This product can be made as a purified or inactivated virus stock, or as purified viral genomic material. Many different reporter proteins and molecular tags can be incorporated. Custom variant design and production services are also available. Please contact us for additional information.

Additional Strains: If you require a strain of this virus not listed on our website, please contact us. We can produce a wide range of common strains to meet your needs.

Additional Viruses Available: If you are looking for a virus species or strain not listed on our website, please contact us with your request. Our team of experts will work with you to create the product that meets your research needs and budget.