Yellow Fever Virus Vaccine Strain 17D mCherry Reporter

Description: This flavivirus expresses the reporter protein mCherry.

Strain History: 17D-204 is the live-attenuated vaccine strain of yellow fever virus (YFV) derived from the virulent human clinical isolate Asibi strain (Ghana, 1927). The genomic sequence is available in NCBI.

Passage History:

• Parental Asibi strain: Rhesus macaque monkeys (53), intermittent passages in Aedes aegypti mosquitoes (Unknown). References (1) and (2).
• 17D-204: Mouse embryo tissue (18), minced whole chick embryo (58), minced whole chick embryo without brain and spinal cord (128). References (3) and (4).

Production: Transfection of in vitro transcribed viral genomic RNA into Vero cells. No serial passages performed.

Biosafety Level: 2

Titer: 3.16 x 10⁶ TCID50/mL on Vero cells.

Format: 1.0 mL/vial. Clarified and filtered cell culture supernatant frozen at -80°C. Delivered on dry ice.

QA/QC:

• Infectious titer in TCID50/mL
• Whole-genome sequencing by NGS
• Mycoplasma test by PCR
• Sterility test for bacterial and fungal contamination
• Endotoxin test <0.25 EU/mL
• Documentation: Experimental conditions for QC tests.

Reporter Virus Construction: Reporter protein coding sequences were inserted between the 5' UTR and the capsid, and contain the first 75 nucleotides of the capsid protein at the 5' end (modified with silent mutations to prevent homologous recombination with the capsid gene) and the 2A-like protease sequence from the Thosea asigna virus (TaV site) at the 3’ end. Upon polyprotein translation in infected cells, the TaV protease cleaves the reporter protein from the capsid, resulting in reporter expression independent of incorporation into the virus particle. For additional information, see reference.

Production Batch Information links (downloadable):

Certificate of Analysis

NGS Results and Complete Genome Sequence

Batch Record: Detailed Production and QC Data

Product Use Policy and Material Transfer Agreement

Additional Formats: This product can be made as a purified or inactivated virus stock, or as purified viral genomic material. Many different reporter proteins and molecular tags can be incorporated. Custom variant design and production services are also available. Please contact us for additional information.

Additional Strains: If you require a strain of this virus not listed on our website, please contact us. We can produce a wide range of common strains to meet your needs.

Additional Viruses Available: If you are looking for a virus species or strain not listed on our website, please contact us with your request. Our team will work with you to create the product that meets your research needs and budget.